This paper describes the results of a survey of the gastro-intestinal helminth parasites in the faecal matters of fourteen wildlife species investigated in five sites in Radom National Park (R.N.P.), South Darfur State, Sudan. These sites were Radom area, Alhufra, Titrbi, Kafindibei and Kafiakingi. Out of 579 faecal matters examined, 177 were found containing eggs of helminth parasites with an overall prevalence of 30.6%. The Aardvark (Orycteropus afer) had the highest overall infection rate of helminth eggs (60%) (9/15), while the Patas monkey (Erythrocebus patas) showed the lowest prevalence (2.7%) (1/37). The helminth parasites recorded throughout this study included Ascaris spp., Trichuris spp., Haemonchus spp., Oesophagostomum spp., Toxocara spp., Trichostrongylus spp., Strongyloides spp., Moniezia spp. and Coccidia oocysts. Kafiakingi area showed the highest prevalence (35.9%) (65/181), followed by Kafindibei area (35.1%) (34/97) while Alhufra area showed the lowest prevalence (17.6%) (13/74). The results of this survey were compared and discussed with previous findings of similar studies conducted in Sudan and elsewhere.