In this study, Indirect Enzyme-linked Immune Sorbent Assay (ELISA) was performed to detect specific antibodies of equid herpes in the North, Middle and South of Syria and determine the serotype of the injury of the typical virus serotypes (1 and 4) in samples of blood collected from 145 horses (horse - mare - foal) non vaccinated against EHV 1/4 spread at three areas, using the enzyme immunoassay test (indirect ELISA). The serotype EHV1 serotype causes abortion in horses It also causes respiratory and neurological disorders, while the EHV4 serotype responsible for respiratory disorders. The prevalence serotype1 (EHV1) in the southern region  4% in the middle region 8%, while in the northern region was 2.2%, The study showed serological prevalence serotype4 (EHV4) in the southern region by 98% in the middle region 88%, while in the northern region was 93.3%.