The present study aimed to investigate the efficacy of laparoscopic intrauterine insemination as compared with cervical insemination in Barki ewes as well as to set the optimum number of spermatozoa per laparoscopic insemination. A total of 33 oestrus-synchronized Barki ewes were divided into four groups, the first three groups were inseminated laparoscopically with either 10 x 10⁷ (group 1), 20 x 10⁷ (group 2) or 40 x 10⁷ sperm/dose(group 3). The 4^th group was cervically inseminated. The results showed that lambing rate after laparoscopic intrauterine insemination using 20 x 10⁷ spermatozoa (75.00%) was superior to either laparoscopic insemination using 10 or 40 x 10⁷ spermatozoa or cervical insemination using 80 x 10⁷ spermatozoa (37.50%, 62.50 and 55.56%, respectively). Conclusively, intrauterine insemination using the technique of laparoscopy is a relatively simple, field applicable and convenient mean of achieving high lambing rates. Furthermore, 20×10⁷ motile spermatozoa is the recommended laparoscopic insemination dose in Barki ewes.