The present study aimed to determine the seroprevalence of brucellosis in the serum of non vaccinated ewes by indirect enzyme-linked immunosorbent assay (I-ELISA), and to investigate possible effect of brucellosis on the serum lipids profiles in infected ewes and find correlation coefficients among all parameters. These study included examination of 120 blood serum samples (collected from non-vaccinated ewes) in different areas of Duhok Governorate, Iraq. Results showed that prevalence of brucellosis(by using I-ELISA) was 39.1% and also showed significant increased in total cholesterol, triglyceride (TG),  in the investigated ewes (one week post abation) low density lipoprotein and high density lipoprotein levels, whereas very low density lipoprotein value is significantly decreased compared with the (negative for I-ELISA)-group.