Larval-stage of the bot-fly Gasterophilus obtained from the stomach of Egyptian donkeys (stomach of the newly dead donkeys in Alexandria Governorate) were studied.  Comparison of cuticular features, including spine distribution and shape, structure of maxillae and mandibles, cephalic sensillae and terminal abdominal segments of third instar of Gasterophilus intestinalis, Gasterophilus haemorrhoidalis,Gasterophilus nasalis and Gasterophilus pecorum.This study also focused on clarifying the fundamental differences between the second and third instars of G. haemorrhoidalis using scanning electron microscopy. One or more features distinguished among the species for the first time in Egypt. Gasterophiline larvae are of veterinary and medical importance with some human creeping cutaneous myiasis, ophthalmomyiasis and one recent record of intestinal myiasis.