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170980

AVIAN INFLUENZA VIRUS AND NEWCASTLE VIRUS SURVEILLANCE AND CHARACTERIZATION IN BROILER AND LAYER CHICKEN FLOCKS IN EGYPT

Article

Last updated: 23 Jan 2023

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Abstract

Considering that highly pathogenic avian influenza H5N1 endemically circulates in the Egyptian poultry population, Active surveillance was undertaken in 195 broiler and layers farms from Eighteen Egyptian governorates resulted in, 34,7 and 24 positive farms for AI H9N2, AI H5N1 and Virulent ND respectively, using RT-PCR. The genetic analysis of avian influenza viruses of HA gene of the H5N1 type revealed that these viruses are highly pathogenic strains as they are tracking clade 2.2.1 of classic viruses which became endemic in  Egypt since 2006. However, H9N2 viruses revealed that they are low pathogenic as they are tracking G1-lineage of Asian viruses which transmitted to Middle East scince 2000. Also sequence analysis for F gene for twelve NDV isolates revealed that they are of virulent type and genetically related to genotype VII NDV except two isolates are avirulent type and resemble Lasota- like- strains. Pathogenecity studies for NDV isolates using ICPI was evaluated for the strain (NDV-EG-567f-2012) which of virulent with ICPI value 1.96. The analysis of isolated strains by different diagnostic and analytical techniques implies that the H5N1 and H9N2 silent infection exist in Egypt.

DOI

10.21608/avmj.2014.170980

Keywords

Key words: Pathogenisity- Lasota like strains- lineage- RT, PCR- endemic

Authors

First Name

AZHAR

Last Name

G. SHALABY

MiddleName

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Affiliation

Reference Laboratory for Veterinary Quality Control on Poultry Production Ministry of Agriculture, P.O. Box 264,Dokki, 12618 Giza, Egypt.

Email

azhar_gaber@yahoo.com

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Orcid

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First Name

AHMED

Last Name

M. ERFAN

MiddleName

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Affiliation

Biotechnology Department, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Nadi El-Seid St., P.O. Box 246, Dokki, Giza 12618, Egypt.

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City

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First Name

FATMA

Last Name

A. ABDEL REHEEM

MiddleName

-

Affiliation

Biotechnology Department, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Nadi El-Seid St., P.O. Box 246, Dokki, Giza 12618, Egypt

Email

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City

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Orcid

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First Name

ABDULLAH

Last Name

A. SELIM

MiddleName

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Affiliation

Poultry Diseases Department, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Nadi El-Seid St., P.O. Box 246, Dokki, Giza 12618, Egypt

Email

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City

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Orcid

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First Name

MOHAMED

Last Name

H. AL HUSSENY

MiddleName

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Affiliation

Biochemistry Department. Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Nadi El-Seid St., P.O. Box 246, Dokki, Giza 12618, Egypt.

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City

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Orcid

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First Name

SHEREEN

Last Name

G. KHOLOSY

MiddleName

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Affiliation

Epidemiology Department, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Nadi El-Seid St., P.O. Box 246, Dokki, Giza 12618, Egypt.

Email

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City

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Orcid

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First Name

SOAD

Last Name

A. NASEF

MiddleName

-

Affiliation

Poultry Diseases Department, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Nadi El-Seid St., P.O. Box 246, Dokki, Giza 12618, Egypt.

Email

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City

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Orcid

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Volume

60

Article Issue

142

Related Issue

24326

Issue Date

2014-07-01

Receive Date

2014-06-16

Publish Date

2014-07-01

Page Start

100

Page End

109

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_170980.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=170980

Order

15

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

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Article

Created At

23 Jan 2023