This study was conducted using Forty five dairy cows selected randomly from small different farms. Blood and milk samples were collected during three stages of lactation (early, middle and late), on each serum sample total proteins, albumin, glucose, urea, total lipids, cholesterol, triglycerides, calcium, phosphorus and magnesium were determined. In each milk sample fat %, total proteins, calcium, phosphorous, and magnesium levels were measured. A significant effect of the lactation stages were observed on glucose, urea, total lipids, cholesterol, triglycerides, and calcium, while there were no significant differences during the three lactation stages on serum total protein, albumin, phosphorus and magnesium. In case of the examined milk samples there were no significant differences in fat %, urea, calcium, phosphorus and magnesium in the different lactation stages. While, there was a significant increase in milk protein in the middle and late lactation stages.