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170949

CHLAMYDIA INFECTION IN ABORTED AND CLINICALLY HEALTHY EWES IN ASSIUT GOVERNORATE USING MICROSCOPICAL EXAMINATION AND ELISA

Article

Last updated: 05 Jan 2025

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Abstract

In 3 sheep breeding flocks in Assiut Governorate suffered from a problem of abortion, after excluding Brucella infection using Rose Bengal and Buffered acidified plate antigen test. Serum and endocervical swabs from 92 ewes (72 with a history of abortion, 20 clinically healthy ewes) were examined using ELISA for detection of antibodies against chlamydia and Giemsa staining for detection of intracytoplasmic inclusion bodies in endocervical swabs. Only 16/72 (22.22%) and 1/20 (5%) of previously aborted and clinically healthy ewes respectively were found to be positive using ELISA, infection were more prevalent in ewes over 5–7 years (29.17 %) than in other age groups. No intracytoplasmic inclusion bodies were detected in endocervical swabs. It could be concluded that chlamydia infection was prevalent in ewes in Assiut Governorate; ELISA was a rapid screening test for detection of infection in ewes while Giemsa staining was not a reliable method in diagnosis of it.

DOI

10.21608/avmj.2014.170949

Keywords

Key words: Chlamydia, ELISA, endocervical swabs, Assiut, IgG immunoglobulins

Authors

First Name

S.M.

Last Name

EL BERBAWY

MiddleName

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Affiliation

Animal Health Research Institute (AHRI), Assiut Lab

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Orcid

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First Name

KHALED

Last Name

A.S. EL-KHABAZ

MiddleName

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Affiliation

Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Assiut University, Egypt.

Email

khaledsayed_1970@yahoo.com

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Volume

60

Article Issue

142

Related Issue

24326

Issue Date

2014-07-01

Receive Date

2014-05-08

Publish Date

2014-07-01

Page Start

23

Page End

28

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_170949.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=170949

Order

4

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

CHLAMYDIA INFECTION IN ABORTED AND CLINICALLY HEALTHY EWES IN ASSIUT GOVERNORATE USING MICROSCOPICAL EXAMINATION AND ELISA

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Article

Created At

23 Jan 2023