Shiga toxins (Stx) are the major virulence factors of STEC, which cause diarrhea, life-threatening haemolytic uraemic syndrome (HUS) and acute renal failure in children. This work aimed to evaluate the new RIDA^®QUICK Verotoxin/O157(R-Biopharm, Darmstadt, Germany), which detects Stx & E. coli serogroup O157and to determine the extent of faecal contamination of meat products by STEC. A total 623 samples, including 214 faecal samples collected from sheep, 209 stool samples from human and 200 samples of different meat products were cultured on Trypticase Soya Broth and CT-Sorbitol MacConkey agar, samples were Biochemically identified as Escherichia coli. Further identifications were performed by serotyping and Vero cells cytotoxicity assay. PCR for specific VT1/VT2 genes and RIDA^®QUICK Verotoxin/O157 were applied on VCA positive samples. The results of RIDA^®QUICKrevealed the detection of STEC in 14(93.33%), 6 (85.71%) and 13(86.66%) while PCRtest detected 15(100%), 7(100%) and 15(100%) VCA positive samples from sheep, man and meat products, respectively. Overall RIDA®QUICK Verotoxin / O157 Combi test detected 33(89.18%) from 37 strains of STEC that were positive for VCA and PCR. The test was sensitive for the Stx1 (9/9 positive), Stx2 (18/22 positive) and Stx1& Stx2 (6/6). Moreover, The sensitivity of the assay for the E. coli O157 antigen was tested with 7 strains belonging to sheep (4 strains) and man (3strains), The RIDA Quick assay detected all of them. Furthermore, Strains belonging to the non-O157 serogroups did not react for the O157 antigen in the RIDA Quick assay indicating a specificity of 100%. In Conclusion: Fecal contamination of meat products is important route through which STEC pathogens enter the food chain and the new RIDA^®QUICK Verotoxin/O157 is a highly sensitive, specific and rapid method to determine the presence of Stx and serogroup O157 in enrichment cultures.