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170038

EFFECT OF MELATONIN ON BUFFALO BULL SPERM FREEZABILITY, ULTRASTRUCTURE CHANGES AND FERTILIZING POTENTIALS

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Last updated: 23 Jan 2023

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Abstract

Cryopreservation induces sublethal damage to the spermatozoa, which leads to reduce their fertile life. The objective of the present study was to investigate the effect of melatonin on freezability, ultrastructure and fertilizing potentials of the buffalo spermatozoa. Buffalo spermatozoa were cryopresreved with Tris egg yolk extender containing 7% glycerol supplemented with (0.100, 0.250, 0.500, 0.750 and 1 mM melatonin) or Tris-based extender only (control). Cryopresreved spermatozoa were assessed for post-thawing sperm motility, viability and acrosomal integrity, ultrastructure changes and fertilizing potentials. The current results clearly indicated that adding 0.1 mM melatonin to Tris extender significantly improved (P<0.05) post-thawing sperm motility and viability index following cryopreservation (60.00±2.9% and 157.5±11.8, respectively) compared with the control spermatozoa (45.00±2.90% and 97.00±9.09, respectively). The current results illustrated that addition of 0.1mM melatonin protected the plasma membrane, acrosomal region and mitochondria and maintained the ultrastructure integrity of the cryopresreved spermatozoa compared with the control spermatozoa. The present data indicated that addition of melatonin at a dose of 0.1 and 0.25 mM to the semen extender significant increased (P< 0.05) the positive HOST percent (83.66 and 81.33%, respectively) compared with the control semen (43.33 %). Additionally, the current results revealed that addition of 0.1 mM and 0.25 mM melatonin to the semen extender highly significant increased (P< 0.01) the pregnancy rate (66.67 and 60.43%, respectively) compared with the control semen (45.65%). Therefore, the present results revealed that addition of melatonin to the freezing extender might improve semen quality and reduce cryodamage of the buffalo bull spermatozoa.

DOI

10.21608/avmj.2015.170038

Keywords

Key words: Melatonin, semen extender, cryopreservation, Host, ultrastructure and conception rate

Authors

First Name

MOHAMED

Last Name

EL-RAEY

MiddleName

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Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Benha University (http://www.fvtm.bu.edu.eg), P.O. 13736, Tokh, Kaliobia, Egypt

Email

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Orcid

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First Name

BADR,

Last Name

M.A.

MiddleName

-

Affiliation

Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Al Haram (P.O.B. 12556), Giza, Egypt

Email

magdybadr69@yahoo.com

City

-

Orcid

-

First Name

ASSI,

Last Name

M. M

MiddleName

-

Affiliation

Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Al Haram, P.O. 12556, Giza, Egypt

Email

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City

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Orcid

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First Name

RAWASH,

Last Name

Z.M.

MiddleName

-

Affiliation

Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Al Haram, P.O. 12556, Giza, Egypt.

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Volume

61

Article Issue

144

Related Issue

24324

Issue Date

2015-01-01

Receive Date

2014-12-10

Publish Date

2015-01-01

Page Start

201

Page End

208

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_170038.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=170038

Order

25

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

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Article

Created At

23 Jan 2023