The objectives of this study were to evaluate the relation between lipid profile and total antioxidant capacity (TAC) and to investigate the changes in some hematological and biochemical parameters in buffalo heifers during pre and post-partum period. The study was conducted on 30 buffalo heifers from a buffalo's farm that belongs to Assiut City, Egypt. These pregnant buffaloes were followed until delivery. Blood samples were taken at 3 weeks pre-partum (late pregnancy) and one month post-partum (early lactation). Two types of samples were collected, whole blood samples for hematological picture, and serum samples for biochemical analysis which included total proteins, albumin, globulins, AST, ALT, triglycerides, total cholesterol, HDL-C, LDL-C, and total antioxidant capacity (TAC). The hematological indices including mean values of RBC, Hb, PCV, MCV, and MCH, TLC, lymphocyte % and neutrophil % revealed significant decrease in the early lactation period if compared with late pregnancy mean values. There were significant increase in total proteins, albumin and globulins in the early lactating group compared with late pregnancy group. The mean values of AST, revealed significant increase in the early lactating group compared with late pregnancy group. A significant decrease in the mean values of triglycerides was observed in the early lactating period compared with late pregnancy results. The mean value of total cholesterol, HDL-C and total antioxidant capacity (TAC) revealed significant increase, in the early lactating group compared with late pregnancy group. There were a positive correlation between Total antioxidant capacity (TAC), albumin, total cholesterol and HDL-C.