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170025

MULTIDRUG RESISTANT BACTERIAL PATHOGENS IN EGGS COLLECTED FROM BACKYARD CHICKENS

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Last updated: 05 Jan 2025

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Abstract

A total of two hundred eggs collected from backyard balady chickens were examined for the presence of Salmonella spp., E. coli, and Coagulase Positive Staphylococci. Out of the total examined samples, no Salmonella was detected. A total of 36 E. coli strains were isolated with an overall isolation rate of 18%. Eighty (40%) Coagulase Positive Staphylococci isolates were detected.  Serotyping of E.coli isolates revealed the predominance of O 27 at a rate of 22% among the 13 identified serotypes. However, 5 (14%) of the E. coli isolates were untypeable. The antibiotic susceptibility pattern of Coagulase Positive Staphylococci was studied against 15 different chemotherapeutic agents. The highest resistance rates were detected against oxytetracyclin, oxacillin and trimethoprim sulphamethazole with resistance rates of 90%, 86.7% and 86.7%, respectively. The highest sensitivity rate was detected towards ampicillin with a percentage of 87%. E. coli isolates were tested for its susceptibility against 14 different antibiotics, the highest resistance rates were recorded against trimethoprim- sulphamethazole, doxycycline, tetracycline and amoxycillin with resistance rates 94.1%, 93.2%, 92.9%, and 92.3%, respectively. While the highest sensitivity rates were detected for colistin, cefotaxim, gentamycin and ciprofloxacin with sensitivity rates of 94.4%, 76.5% 59.8% and 55.6%, respectively. The antibiotic susceptibility results were judged by PCR, for E. coli strains, the blaTEM, sul1 and tetA(A) genes were tested for the ß-lactams, trimethoprim sulphamethazone and tetracyclines, respectively. While the mecA, blaZ and tetK genes were tested for the evaluation of the susceptibility of Coagulase Positive Staphylococci against oxacillin, ß-lactams, and tetracyclines, respectively. E. coli virulence was tested by PCR through the testing of iss, eaeA, stx1, stx2, tsh and pap < /em>C genes. While the virulence of Coagulase Positive Staphylococci was estimated through testing of the enterotoxins genes in addition to coagulase, spa and hlg genes.

DOI

10.21608/avmj.2015.170025

Keywords

Key words: MDR bacteria, E.coli, PCR, balady chicken eggs

Authors

First Name

SAMAH

Last Name

EID

MiddleName

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Affiliation

Bacteriology Dept Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Nadi El-Seid St., P.O. Box 246, Dokki, Giza 12618, Egypt.

Email

samaheid@ymail.com

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Orcid

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First Name

SOAD

Last Name

A. NASEF

MiddleName

-

Affiliation

Poultry Diseases Dept., Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Nadi El-Seid St., P.O. Box 246, Dokki, Giza 12618, Egypt.

Email

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City

-

Orcid

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First Name

AHMED

Last Name

M. ERFAN

MiddleName

-

Affiliation

Biotechnology Dept. Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Nadi El-Seid St., P.O. Box 246, Dokki, Giza 12618, Egypt.

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Volume

61

Article Issue

144

Related Issue

24324

Issue Date

2015-01-01

Receive Date

2014-10-28

Publish Date

2015-01-01

Page Start

87

Page End

103

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_170025.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=170025

Order

12

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

MULTIDRUG RESISTANT BACTERIAL PATHOGENS IN EGGS COLLECTED FROM BACKYARD CHICKENS

Details

Type

Article

Created At

23 Jan 2023