The bacteriological examination of 200 meat samples obtained from diarrheic buffalo calves revealed that Salmonella isolates could be recovered with an incidence 2 %. Serological identification of Salmonella species by using O and H antisera revealed that the most common Salmonella serovars were Salmonella typhimurium followed by Salmonella dublin and Salmonella anatum with an incidence 1 %, 0.5% and 0.5 % respectively. Salmonella typhimurium is inactivated by 3 KGy dose of irradiation of meat and also the isolated organism for preparation of the vaccine. The result of vaccination inoculation challenge studies revealed that the prepared Salmonella typhimurium vaccine was effective in protecting rabbits from salmonellosis.