İn this study the efficiency of four types of routinely used commercial disinfectant and antiseptics (Ethanol 70%, Dettol –Chloroxylenol- 5%, Hibitine -Chlorohexidine gluconate- 6% and Bleach (Sodium hypochlorite 10%) which used in the laboratory of microbiology in College of veterinary medicine, Mosul, Iraq were tested against four different bacterial strains which isolated from clinical specimens of infected animals (E.coli, Pseudomonas aeruginosa, Staphylococcus arueus and Corynebacterium renale). Antibiotic sensitivity tests were applied for different nine antibiotics (Ampicilin, Ciprofloxacin, Gentamycin, Cefotaxim, Cephalothin, Lincomycin, Polymyxin-B, Trimethoprim Sulphamethaxazoll and Penicillin), all the tested bacteria showed resistence for (Ampicilin, Gentamycin, Cefotaxim, Cephalothin, Lincomycin, Trimethoprim Sulphamethaxazoll and Penicillin). Broth dilution method used for determination of minimum inhibitory concentration (MIC) and disc diffusion method, the results of MIC method after 5 minutes of the exposure to the different concentration of Ethanol, Dettol, Hibitine and Bleach showed that Dettol has no effecincy, followed by Hibitine and Ethanol which showed lower activity while Bleach was the most effective disinfectant on the all tested bacteria. The Gram positive bacteria tested in this study were more sensitive than Gram negative bacteria to all used disinfectant and antiseptics, Disc and diffusion methods had similar effectivness for the tested bacteria against the disinfectants and antiseptics that used in this study.