In our investigation phenotypic and genotypic characterization were carried out for detection of Gallibacterium anatis in chickens and ducks that suffered from septicemia, perheptitis, percarditis, trachitis and penumonia. Prevalence of Gallibacterium anatis was 24% and 26% from diseased chickens and ducks respectively, The biochemical investigations differentiated the isolated strains into two starins Gallibacterium anatis biovar heamolytica with a percentage 24 % and Gallibacterium anatis biovar anatis 76 %. Gallibacterium anatis biovar heamolytica represented 30.7% in ducks and 16.6% in chickens while Gallibacterium anatis biovar anatis was represented 69.2% in ducks and 83.3% in chickens. Antimicrobial susceptibility test insured the multidrug resistant of Gallibacterium anatis isolates. PCR technique confirms isolation percentage. Also virulence genes were examined like cytotoxic genes (gtx A1 and A2 and its results were relevant to the phenotypic virulent characteristics in addition to fimberial gene (flfA.). In conclusion some virulent genes like gtxis responsible for evoking the phenotypic character of strain such as hemolysis.