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170234

GENETIC CHARACTERISTICS OF INFECTIOUS BURSAL DISEASE VIRUS IN EGYPT FROM 2012 TO 2014

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Last updated: 23 Jan 2023

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Abstract

The infectious bursal disease virus (IBDV) is a continuing serious problem facing poultry industry in Egypt.  In this study, 24 bursae samples collected from different broiler flocks (from Giza, Dakahleya, Ismailia, Damietta and Alexanderia) experiencing high mortality rate and bursal lesions during 2012 to 2014 were examined. Clear differences in pathotyping between very virulent Infectious bursal disease (vvIBD) and classical IBD were observed in pathological examination. Reverse transcriptase polymerase chain reaction (RT-PCR) was applied for IBD targeting hyper variable region (HVR) of VP2 gene. Genetic analysis revealed that 15 viruses belonged to vvIBD that had amino acids A222, I256, I294 and S299 which are highly conserved among vvIBD starins, while 9 viruses belonged to classical IBDV (similar to vaccine strains). The genotyping of Egyptian vvIBDV indicate progressive evolution compared with previously isolated strains which indicates persistence of vvIBDV in Egyptian poultry environment.

DOI

10.21608/avmj.2015.170234

Keywords

Keywords: IBDV, RT-PCR, HVR, VP2 gene, Pathotyping

Authors

First Name

NAGLAA

Last Name

HAGAG

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Affiliation

National Laboratory for Veterinary Quality Control on Poultry Production, P.O. Box 264, Dokki, Giza.

Email

naglaavetrose@yahoo.com

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First Name

MOHAMED

Last Name

A. SOLIMAN

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Affiliation

National Laboratory for Veterinary Quality Control on poultry production NLQP, P.O.Box264, Dokki, 12618, Giza, Egypt.

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First Name

ABDEL SATAR

Last Name

ARAFA

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Affiliation

National Laboratory for Veterinary Quality Control on poultry production NLQP, P.O.Box264, Dokki, 12618, Giza, Egypt.

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-

Orcid

-

First Name

ALI

Last Name

ZANATY

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-

Affiliation

National Laboratory for Veterinary Quality Control on poultry production NLQP, P.O.Box264, Dokki, 12618, Giza, Egypt.

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Orcid

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First Name

AHMED

Last Name

M. ERFAN

MiddleName

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Affiliation

National Laboratory for Veterinary Quality Control on poultry production NLQP, P.O.Box264, Dokki, 12618, Giza, Egypt.

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City

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Orcid

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First Name

MOHAMED

Last Name

K. HASAN

MiddleName

-

Affiliation

National Laboratory for Veterinary Quality Control on poultry production NLQP, P.O.Box264, Dokki, 12618, Giza, Egypt.

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Orcid

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Volume

61

Article Issue

147

Related Issue

24321

Issue Date

2015-10-01

Receive Date

2021-06-22

Publish Date

2015-10-01

Page Start

43

Page End

50

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 61_Issue 147_Pages 43-50.pdf

PDF . 867.4kB

Link

https://avmj.journals.ekb.eg/article_170234.html

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https://avmj.journals.ekb.eg/service?article_code=170234

Order

6

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

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Article

Created At

23 Jan 2023