As rice production continues to rise in order to provide a stable food source for over half of the world's population, so rice straw will continue to be an abundant and accessible agricultural waste. Rice straw is a widely available lignocellulosic waste with potential for energy recovery through anaerobic digestion. Lignin slows the hydrolysis phase, resulting in low methane recovery and long digestion periods. Pretreatment with wet milling was tested in contrast with grinding rice straw and control one without rice straw, as a technique for enhancing the anaerobic digestion. Two mixtures were used, M1 containing grinded rice straw and M2 containing wet milled rice straw and the both were inoculated with seed sludge and kept under thermophilic conditions (55°C) with dry fermentation (total solids, TS%) over 20. Control vials were used with only seed sludge. Two batch cultures lasted for 35 days for each. The results showed that wet milling seems to enhance methane production from rice straw with maximum production of 1493.98 ml/g-vs compared with 387.7 ml/g-vs from grinded one. Methane percentage was ranged from 48 to 55%. Acetate production was low ranged from 0.74 to 0.93mM/kg mixture which meant success of methane production. Thermophilic condition was used at 55°C which in contrast to previous study enhance the degradation. Methane potential from grinded rice straw, wet milled rice straw, and glucose as reference was 234, 230, and 338 l/kg vs respectively.