Mycotoxins consider as toxic secondary metabolites secreted from fungi which grow in certain condition of temperature and humidity. Now in our area, there is continuous change in climatic condition from year to another so we need to monitor level of mycotoxins in poultry ration regularly. Forty five samples of poultry rations collected from broiler chicken farms of Dakhalia Governorate and were analyzed by HPLC-UV. The present study showed that incidence of aflatoxin B1 (AFB1) and ochratoxin A were 64% and 50 % in starter ration respectively and in finishing one were 52 % and 45% for both aflatoxin B1 and  ochratoxin A respectively. The concentration of aflatoxin B1 on poultry ration under analysis ranged from 0.5 to 62 ppb while ochratoxin A level ranged from 3 to 52 ppb. Additionally, the mean values of aflatoxin B1 and ochratoxin A in strater ration were 17.22 ±20 and 22.9 ±17.33 respectively and in finishing ration were 9 ± 10.04 and 13.8 ±13 respectively. In other word, these value of both mycotoxin is quite similar or low than the maximum european permissible limit of aflatoxin B1 (20 ppb) and for ochratoxin A (100 ppb).