The objective of this study was to provide a detailed laparoscopic anatomy of the abdominal regions and to describe Laparoscopic Clipping Release (LCR) in situ castration technique in lateral recumbent male donkey. Nine adult clinically healthy donkeys were used for the study. Using only two laparoscopic ports and titanium clips to induce in situ castration in lateral recumbent generally anesthetized patients was the golden change out of previous laparoscopic trials. The main port placed 5-10 cm caudal to the umbilicus and secondary port placed in groin region 10 cm dorsal and 10 cm caudal to the main port. Two clips over the course of the vas deference and four clips over the course of the testicular blood vessels (one pair dorsally and one pair ventrally) are fixed using clip applier. This technique provided an optimal access, easy manipulation and handling of testicular structures, short operative time, no intra or post-operative complications and excellent cosmetic appearance. We concluded that LCR technique in right lateral recumbent position is proved to be feasible, safe, less time consuming, and could be brought into consideration as an alternative to in situ equine castration methods.