Through this study, sonicated sporulated Eimeria acervulina oocysts were used for chicken immunization purpose. A total number of 80 susceptible chicks were divided into four groups (A, B, C and D, 20chicks/ each), the first three groups received sonicated sporulated oocysts of Eimeria acervulina in dose of 30000, 40000 and 50000 per os /one chick respectively while group D was left without immunization as a control group. The first three groups with stand challenge test using hot strain of Eimeria acervulina and showed protection percentage of 70%., 80% and 85% respectively with minimal lesion score, obvious increase  body weight gain and good health condition compared with the control group  which recorded high level of score lesion up to +4 with 0% protection and high mortality rate. Also there was observed reduction in fecal oocysts shedding in immunized chicks compared with the control one. On applying ELISA., it was found that there was increasing in antibody titer against Eimeria acervulina in immunized birds compared with the control group. S0 it could be corcluded that 50000 sonicated oocyct represent the best protective dose protected chicks against E.acervulina infestation.