This study was conducted for isolation and identification of staphylococcus aureus from minced meat and evaluation of sensitivity of these strains to antibiotics. Also, this study aimed to assess the activity of Iraqi ethanol extract of propolis (EEP) against S.aureus compared to European ethanol extract of propolis at 10%, 15%. 20% and 30% to both types of propolis. Thirteen samples of minced meat revealed positive results for isolation of S.aureus with a ratio of (21%). Iraqi EEP showed antibacterial activity against S.aureus at 3 µg with average of inhibition zone 18.84±0.256 compared to European EEP which gave inhibition zone against the growth of S.aureus 12.84±0.354 at same concentration. Other concentration of EEP gave low sensitivity against S. aureus strains which mean that Iraqi EEP have a high activity against these strains related to flavonoides and phenols activity presented in Iraqi EEP. All strains of S.aureus isolated from minced meat showed high sensitivity to Cephtriaxon at 10 µg while all of them showed high resistance to Methacillin, Gentamycin and Penicillin at 10 µg concentration.