A total of 60 random samples of meat were collected from different butchers shops at Port- Said city and subjected to bacteriological examination. The prevalence of Staph. aureus coagulase positive was 10 (16. 6%). The coagulase positiveStaph. aureus isolates were tested for their susceptibility to methicillin/ Oxaciillin. 6(60%) of the isolates were resistant to methicillin/ Oxaciillin. Multidrug resistance of MRSA strains was observed. The maximum resistance was observed against Ampicillin and Chloramphenicol (100%) while the least resistance was observed against Streptomycin (66.6%) followed by Gentamicin and Trimethoprim + Sulphamethoxazole (33.3%) and Erythromycin (16.6%). The isolates were analyzed for the presence of the genes encoding resistance to methicillin (mecA) by using Polymerase chain reaction (PCR) which is a powerful and a rapid procedure for the detection of mecA gene in methicllin-resistance (MRSA) strains. Only 3 out of 6 isolates were found to carry the mecA gene amplified at 310 bp. The Staph. aureus found in meat directly affect the human health and can cause a public illness if the meat is used in the food industry. Examination of meat using convetional PCR is a useful technique for detection of mecA gene inMethicillin-resistanceStaph. aureus (MRSA).