Trace elements deficiency constitutes an economic problem, can seriously threaten a large scale of the ruminant. It resulted in hemato-biochemical alterations which adversely impact animal's production. The present study was designed to investigate factors affecting animal's minerals status and evaluate correlation attributed element deficiency. This study was established on 300 sheep including lamb and adults from both sexes (male and female), animals comprising healthy and diseased sheep. Blood samples were collected for hematological and biochemical determination at winter and spring seasons of year 2015 and 2016 in Qena governorate. It could be concluded that several factors can affect trace elements deficiency like sex, age, lactation, pregnancy and healthy status of the animals. Diseased sheep which showed signs of alopecia, dehydration and anemia are suffered from serum elements deficiency like total iron, zinc, calcium, phosphorus and magnesium, besides reduction in red blood cell count and hemoglobin concentration.