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169230

OCCURRENCE OF CAMPYLOBACTER SPECIES IN CHICKENS BY MULTIPLEX POLYMERASE CHAIN REACTION

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Last updated: 05 Jan 2025

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Abstract

The genus Campylobacter is one of great importance to public health because it includes several species that may cause diarrhea. Poultry and poultry products are known as important sources of human campylobacteriosis. 225 samples were collected from (75) chickens including intestinal content (75), liver (75) and skin (75). The overall occurrence of Campylobacter jejuni and Campylobacter coli in chicken by PCR were (5.3% & 17.8%). Multiplex PCR targeting 23S rRNA specific for genus Campylobacter, hip O gene specific for C. jejuni and glyA gene specific for C. coli was used for the confirmation of phenotypically identified C. jejuni and C. coli isolates. It is concluded that PCR was determined to be more specific and rapid than biochemical tests.  

DOI

10.21608/avmj.2017.169230

Keywords

Campylobacter, chickens, Multiplex PCR

Authors

First Name

ASMAA

Last Name

GAHLAN YOUSEEF

MiddleName

-

Affiliation

Zoonoses Department, Faculty of Veterinary Medical, South Valley University

Email

asmaagahlan@yahoo.com

City

-

Orcid

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First Name

A.I.

Last Name

IBRAHIM

MiddleName

-

Affiliation

Poultry Diseases Department, Faculty of Veterinary Medical, South Valley University

Email

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City

-

Orcid

-

First Name

AMAL

Last Name

SAYED

MiddleName

-

Affiliation

Animal Hygiene and Zoonoses Department, Faculty of Veterinary Medical, Assiut University.

Email

-

City

-

Orcid

-

First Name

MONA

Last Name

SOBHY

MiddleName

-

Affiliation

Reproductive Diseases Department, Animal Reproduction Research Institute, El-Haram, Giza

Email

monagabr17@yahoo.com

City

-

Orcid

-

Volume

63

Article Issue

152

Related Issue

24316

Issue Date

2017-01-01

Receive Date

2016-12-14

Publish Date

2017-01-15

Page Start

66

Page End

72

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_169230.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=169230

Order

9

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

OCCURRENCE OF CAMPYLOBACTER SPECIES IN CHICKENS BY MULTIPLEX POLYMERASE CHAIN REACTION

Details

Type

Article

Created At

23 Jan 2023