The present study was conducted on 150 ducks collected from ten farms in Kaliobia Governorate suspected to be suffering from Pasteurellosis that is manifested by respiratory signs, sudden death, and nervous manifestation. Samples were taken from the liver, spleen, heart and lung and examined bacteriologically. A total of 33 Pasteurella isolates were isolated, 25 isolates were Pasteurella multocida and 8 isolates were Pasteurella pneumotropica. The invitro-antibiotic sensetivity test showed that Pasteurella isolates were highly sensitive to florofinicole followed by ciprofloxacine and enrofloxacin. Meanwhile, the isolates showed absolute resistance to erythromycin followed by gentamycin, amoxicillin, oxytetracycline, penicillin, tobramycin and naldixic acid for both types of Pasteurella. PCR results showed that cytotoxic protein (toxA) gene and fimbrial protein (ptfA) gene were detected in 4 out of 10 studied strains for both genes.  Sequences of toxA and ptfA genes were submitted to Gen Bank and assigned accession numbers were MF167359 and MF382009, respectively.