The main goal of this study was check of antigenic drift from field isolates with regard to the international reference strain Colorado and ascertains whether the viral infection can be controlled by the vaccines. To achieve that, the BoHV-1 isolates were isolated with antigenic and genetic characterization and Sodium Dodecyl Sulphate-Polyacrylamide Gel electrophoresis (SDS-PAGE) the soluble proteins of these isolates with dendrogram analysis the results. From 3 different farms at Delta regions, Egypt, samples were collected From the 1st farm, 3 vaginal swabs represented 17 diseased cattle suffer from pustular vulvovaginitis, from the 2nd farm, tissues (liver, spleen and lung) of 2 aborted feti at 5 and 6 months of gestation and from the 3rd one, 3 nasal swabs of recovery cattle from respiratory manifestations after treated with antibacterial, antipyretic and non steroid anti-inflammatory were collected and processed. The samples were tested by direct fluorescence antibody (FA), nested PCR (nPCR), and isolated on Madin-Darby Bovine Kidney (MDBK) cellular culture and dendrogram analysis of electrophoretic pattern the soluble proteins from field isolates. Five samples (3 vaginal swabs and tissues of 2 aborted feti) were showing fluorescence granules. The DNA of the 5 FA positive samples was amplified within gB gene and yield amplicons sized 294 bp. The viruses from the 5 FA and PCR positive samples were isolated and propagated to antigenic and genetic characterization. To achieve that and at the third passage on MDBK cell culture, clear cytopathic effects (CPEs) were seen. Concerning to the dendrogram analysis, overall homology between the different field isolates and the reference international strain (Colorado) was 74-90% and among the field isolates was 81 to 94%. In conclusion, overall homology between the different Egyptian isolates and the reference international strain (Colorado) and among the Egyptian isolates was high. This can help to reduce the scale of future BoHV-1 infections and reduce the extent of culling or emergency vaccination needed.