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170941

STUDY ON SOME ENTEROPATHOGENS OF STREET VENDED MEAT MEALS

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Last updated: 23 Jan 2023

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Abstract

Sixty random samples of street vended meat meals including 20 samples each of Shawerma, Liver sandwich and Hawawshi were randomly collected from different street vendors at Damanhur city Elbehaira Governorate for chemical and microbiological evaluation. The results revealed that, the mean values of total volatile nitrogen (TVB-N), Thiobarbituric acid (TBA) and Aerobic Plate count (APC) were 27.8±0.48, 25.8±0.17, 27.6±0.41 mg/100gm; 0.71±0.02, 4.2±0.33, 0.73±0.01mgMD/kg and 7.5x104±0.85x104, 4.8x104±0.50x104, 4.5x104±0.48 x104cfu/g in the examined samples of Shawerma, liver sandwich and Hawawshi, respectively. Also, enterococci, enteropathogenic E.coli, Salmonella and Shigella were isolated with an incidence of 25, 20, 25 %; 30, 20, 25 %; 10, 15, 0 % and 15, 10,10% from the examined samples of Shawerma, liver sandwich and Hawawshi, respectively. Serotyping of the obtained isolates of enteropathogenic E. coli revealed the detection of O157: H7, O128: K67, O86: K61, O26: K60 and O26: K72 strains at varying rates, Stereotyping Salmonella isolates revealed the detection of S. Enteritidis, S. Typhi and S. Paratyphi. In addition to, biochemical identification of the obtained Shigella isolates revealed the detection of S. dysenteriae, S. flexneri and S. sonnei. Two strain of isolated E.coliO157:H7 and one salmonella isolates were investigated by using PCR to detect presence of virulent genes stx1 (614 bp) and stx2 (779 bp) in E.coli O157:H7 and stn. Gene (617 bp) in Salmonella. From previously mentioned results, enteric pathogens still constitute common contaminants of street vended meat meals and their presence was very important due to its public health significance therefore strict hygienic measures must be applied to obtain safe and wholesome street vended meat meals.  

DOI

10.21608/avmj.2017.170941

Keywords

Key words: Meat meals, TVB-N, tba, E.coli, O157:H7 Salmonella, Shigella

Authors

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SABER,

Last Name

A.S.

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Affiliation

Researcher of Food Hygiene Unit, Animal Health Research Institute, Damanhur Branch, Egypt.

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First Name

HANAA,

Last Name

ELHOFY

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Affiliation

Senior Researcher of Biochemistry Unit, Animal Health Research Institute, Damanhur Branch, Egypt

Email

ahmadbkheet@yahoo.com

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First Name

SALWA,

Last Name

HENDAWY

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Affiliation

Researcher of Food Hygiene Unit, Animal Health Research Institute, Damanhur Branch, Egypt

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Volume

63

Article Issue

155

Related Issue

24313

Issue Date

2017-10-01

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2017-09-13

Publish Date

2017-10-17

Page Start

24

Page End

35

Print ISSN

1012-5973

Online ISSN

2314-5226

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https://avmj.journals.ekb.eg/article_170941.html

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https://avmj.journals.ekb.eg/service?article_code=170941

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Publication Title

Assiut Veterinary Medical Journal

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https://avmj.journals.ekb.eg/

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Created At

23 Jan 2023