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168931

CONVENTIONAL AND MOLECULAR DETECTION OF MYCOBACTERIUM BOVIS IN ABURDEN ANGUS CATTLE AND HUMAN CONTACT IN THE NEW VALLEY GOVERNORATE, EGYPT

Article

Last updated: 05 Jan 2025

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Abstract

In this work, Mycobacterium bovis isolated and identified by microbiological culture and RT-PCR from emergency slaughtered aburden Angus cattle suffering from chronic mastitis and respiratory manifestations in private farm not perform tuberculin test in New Valley governorate, Egypt. Furthermore, other contact cattle, sheep and human in the farm examined by ELISA, found that 22.2% (4/18) cattle in the farm were positive, with complete absence of infection in the sheep and contact persons in the farm. The high percent of M. bovis was detected by RT-PCR (83.33%) followed by microbiological culture (72.22%) in slaughtered cattle. The highest percent of tubercles lesion observed in thoracic cavity followed by the lung then the udder. From our study we can concluded that, M. bovis isolated and identified from imported Aburden Angus cow in New Valley Governorate. Also, ELISA test need further evaluation before using as an exploratory test for diagnosis of BTB in animal and human.

DOI

10.21608/avmj.2018.168931

Keywords

Key words: Post mortem inspection, Bovine tuberculosis, RT-PCR, ELISA

Authors

First Name

YASSER

Last Name

ELNAKER

MiddleName

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Affiliation

Department of Animal Medicine (Infectious Diseases) Faculty of Veterinary Medicine, New Valley, Assiut University, Egypt

Email

yasserelnaker@yahoo.com

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First Name

NAGWA

Last Name

ELSHARAWY

MiddleName

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Affiliation

Dept. of Food Hygiene Fac. Vet. Med., New Valley, Assiut Univ, Egypt

Email

dr.nagwa2004@yahoo.com

City

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First Name

MOHAMED

Last Name

DAIB

MiddleName

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Affiliation

Department of Animal Hygiene and Zoonoses, Fac. Vet. Med., New Valley, Assiut Univ, Egypt.

Email

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City

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Orcid

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First Name

ATTIA

Last Name

ELGEDAWY

MiddleName

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Affiliation

TB Unit, Animal Health Research Institute, Dokki, Egypt.

Email

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City

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Orcid

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First Name

NERMIN

Last Name

IBRAHIM

MiddleName

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Affiliation

Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Mansoura University, Egypt.

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Volume

64

Article Issue

157

Related Issue

24311

Issue Date

2018-04-01

Receive Date

2018-03-08

Publish Date

2018-04-01

Page Start

179

Page End

186

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_168931.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=168931

Order

21

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

CONVENTIONAL AND MOLECULAR DETECTION OF MYCOBACTERIUM BOVIS IN ABURDEN ANGUS CATTLE AND HUMAN CONTACT IN THE NEW VALLEY GOVERNORATE, EGYPT

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Article

Created At

23 Jan 2023