Two hundred fifty samples from different broiler chicken flocks were subjected for isolation of salmonella species (2017-2018). The percentage for isolated Salmonella was 14.4% (36/250). The obtained Salmonlla strains were obtained (Salmonella bardo, Salmonella norwich, Salmonella brancaster, Salmonella sekondi Π, Salmonella lamberhurst, Salmonella belgdam, Salmonella kentucky, Salmonella enteritidis, Salmonella goetebory, Salmonella kisii, Salmonella nigeria, Salmonella grampian,  Salmonella newport, Salmonella noyo, Salmonella colindale, Salmonella seremban, Salmonella remo, Salmonella lindenburg, Salmonella a natum, Salmonella virchow, Salmonella tamiland, Salmonella york. In vitro antimicrobial sensitivity testing carried out on isolated salmonella strains revealed different antimicrobial resistance variation, high resistance rate were observed with lomefloxacin (77.7%), tetracycline (61%), kanamcin (50%) and trimethoprime and levofloxacin (47.2%). Also strains were subjected for detection of biofilm formation using glass tube test and detection of fimA gene was used for biofilm confirmation, 61.11% (22/36) of strains was having ability to produce biofilm, while 38.88 % (14/36) have no ability for biofilm production. Both positive and the negative biofilm formation of salmonella strains revealed the same degree of antibiotic resistance (100%). No great significance between biofilm formation, multidrug resistance and the intensity of clinical signs and postmortem lesions were observed, so no relation between biofilm formation and antimicrobial resistance.