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168956

MYCOPLASMA OVIPNEUMONIA: ISOLATION AND MOLECULAR IDENTIFICATION IN DISEASED SHEEP FLOCK IN DELTA REGION, EGYPT

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Last updated: 23 Jan 2023

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Abstract

Mycoplasma ovipneumoniae (M. ovipneumoniae) was isolated from nasal swabs obtained from sheep with respiratory manifestations in delta region, Egypt; wherein 31 sheep with different ages were suffer from nasal discharge, cough, pneumonia, keratoconjunctivitis in a flock of sheep containing 134 sheep. By mycoplasma culture, nine samples were positive from 31examined samples (29%). M.ovipneumoniae is detected in 33.33% (3 out of 9 isolates of mycoplasma) using PCR with specific primer. The high percentage of isolation found in more than one-year age group, strains were isolated from the nasal swabs and no detection from ocular swabs. Isolated M. ovipneumoni strain subjected to sequencing and was designated as NMD-EG016, which showed a 94.6% 16S-23S RNA intergenic spacer sequence identity with three USA strains (M. ovipneumoniae-2014-2278-10, Mycoplasma.sp. clone-10OR05 and M.ovipneumoniae-1992-6751-17) and >94.4with standard strain ATCC 29419. 

DOI

10.21608/avmj.2018.168956

Keywords

Key words: Mycoplasma ovipneumoni, sheep, PCR

Authors

First Name

NERMIN

Last Name

IBRAHIM

MiddleName

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Affiliation

Department of Bacteriology, Mycology and immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt 35111

Email

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Orcid

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First Name

M.A.

Last Name

EL BESKAWY

MiddleName

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Affiliation

Department of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt 35111. Animal Medicine

Email

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City

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Orcid

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First Name

DINA

Last Name

ELSHAFAY

MiddleName

-

Affiliation

Mycoplasma Department, Animal Health Research Institute, Dokii, Giza

Email

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City

-

Orcid

-

First Name

AMANY

Last Name

ABD-ELMOATY

MiddleName

-

Affiliation

Department of Bacteriology, Mycology and immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt 35111.

Email

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City

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Orcid

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First Name

YASSER

Last Name

ELNAKER

MiddleName

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Affiliation

Department of Animal Medicine (Infectious Diseases) Faculty of Veterinary Medicine, New Valley, Assiut University, Egypt

Email

yasserelnaker@yahoo.com

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Orcid

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Volume

64

Article Issue

158

Related Issue

24310

Issue Date

2018-07-01

Receive Date

1999-11-30

Publish Date

1999-11-30

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ168956943912800.pdf

PDF . 487.7kB

Link

https://avmj.journals.ekb.eg/article_168956.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=168956

Order

9

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

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Article

Created At

23 Jan 2023