The study was conducted in 3 farms in Behera, Matrouh and Dakahlya provinces. These organized farms did not have previous records on animals with confirmed M. bovis infection as the herd had been previously skin tested. A total of 1323 animals of 1 year old and above were included in the study. All animals under our experiment were examined using Tuberculin Test; the positive cases were examined using T.B. Feron Test. The positive cases in both tests were slaughtered for PM and some samples were taken for bacteriological examination on specific media. Confirmation of the isolates by real time PCR. The results revealed that the number of total tested cattle by tuberculin were 1323 the positive were 357 (27 %) by tuberculin test, then tested by bovine gamma-interferon (γ-IFN) enzyme immunoassay, the positive were 212 (16 %), in the first farm the tested cattle were 120 animals and the positive were 79 (6 %) by tuberculin test, then tested by bovine gamma-interferon (γ-IFN) enzyme immunoassay, the positive were 46 (3.5 %), the second farm the tested cattle were 520 and the positive reactors for tuberculin test were 274 (20.7 %), then tested by bovine gamma-interferon (γ-IFN) enzyme immunoassay, the positive were 166 (12.5 %) and the third farm the tested cattle were 683 and the positive cases were 4 animals (0.3 %) by tuberculin test, then tested by bovine gamma-interferon (γ-IFN) enzyme immunoassay and all the samples gave negative results. It is important to increasingly focus resources to target control strategies based on more effective diagnostic methods so the usage of T.B. Feron for detection of T.B. is more useful. And also the usage of T.B. Feron test to differentiate between vaccinated and infected cattle.