The objectives of this study were to record the changes in some acute phase proteins (APPs) either positive or negative during transition period and to demonstrate the correlation between different APPs in the transition period. To achieve these objectives, 29 pregnant buffaloes were selected at 2^nd and 3^rd milk season from Ard El -Kheir farm. The study was carried out in Assuit Governorate, Whole blood samples were collected from these buffaloes for determination of fibrinogen, blood plasma samples were collected for determination of vit. A and blood serum samples were collected for determination of serum albumin, serum total cholesterol, serum total bilirubin and serum haptoglobin). Blood samples were collected as the following: (-21d: -15d), (-14d: -8d), (-7d: -1d), calving day, 3 d P.P, 7 d P.P, 14 d P.P and 28 d P.P.  The study revealed significant changes in the concentration of some acute phase proteins, whether positive or negative. The mean values of fibrinogen revealed significant changes with maximum increase at day 3 P.P. Non significant changes in the mean values of haptoglobin with maximum increase one week pre partum. Significant increase in the mean values of total bilirubin at calving day and post partum periods compared with pre partum periods, reached maximum at day 14 P.P. The mean values of albumin revealed significant changes with maximum increase one week pre partum. A significant decrease in the mean values of total cholesterol at pre partum compared with post partum reached maximum at calving day compared with pre and post partum periods. A significant decrease in the mean values of vit. A reached maximum at day 7 P.P.