Migratory birds play a significant role in the ecology, circulation and transmission of zoonotic pathogens specially Salmonellathat producing animal and human illnesses in addition to sever economic losses to poultry industry, so that the prevalence of Salmonella was estimated and studied from six species of migratory waterfowl birds along Manzala Lake in Dakahlia and Damietta Governorates, Egypt during the period from November 2017 to March 2018. A total of 100 live birds were collected from hunters and the internal organs of those birds were subjected to bacteriological examinations, antimicrobial susceptibility testing and molecular detection of some virulence genes. Seven Salmonella isolates were isolated with a percentage of 7%, (7 out of 100) from Common Teal, Mallard ducks and Shoveler birds. Four different serotypes across the study sites were identified; S. Typhimurium, S. Bardo, S. Montevideo and S. Kentucky. All Salmonella isolates showed high sensitivity to Streptomycin, Erythromycin, Norfloxacin, Colistin sulphat and Doxycycline. Two S. Bardo isolates,                  S. Typhimurium, S. Kentucky showed multi- drug resistance. S. Montevideo and 2 S. Bardo isolates showed higher sensitivity to most of the used antimicrobial agents. Seven virulence genes (invA, sop < /em>B, mgtC, bcfC, spvC, fimH and fimA) were detected using Polymerase Chain Reaction technique (PCR) in all of the examined Salmonella isolates.