In the present study, a total of 160 samples (lung, air sacs, liver and kidneys, 40 samples each) were collected from clinically diseased broiler chickens. Clinical signs were weakness, gasping, ruffled feather, nasal mucoid discharge, poor growth, facial oedema and conjunctivitis, while postmortem findings were signs of septicemia, pneumonia, air sacculitis, nephritis, sinusitis and liver and lung abscesses. Isolation and biochemical identification of K. pneumoniae were done. Results of bacteriological examination revealed that K. pneumoniae isolates were recovered from 160 samples with overall prevalence (14.4%). The isolation rates from lung, air sacs, liver and kidneys were 30 %, 12.5%, 10% and 5%, respectively. The isolates of K. pneumoniae were found to be virulent by using PCR assay incorporating magA, fimH and traT genes primers and were found to be resistant to some antibiotics by using PCR assay incorporating tetA(A), blaTEM and mphA genes primers. Antibiogram for 20 recovered K. pneumoniae isolates against 10 commercially used antibiotics in broiler chicken farms revealed that K. pneumoniae isolates were completey resistant to oxytetracycline (100%) and ampicillin (100%) followed by erythromycin (90%), streptomycin (80%), cefotaxim (70%) and gentamycin (65%) and moderate resistance to neomycin (45%) and chloramphenicol (30%). On the