To evaluate the effect of PRP and insulin on promotion of healing rate in experimental animal's long bone fracture. The current study was conducted with 27 white albino rats weighing between 150 and 200 gm. These animals were divided into three groups (nine rats in each). The experiment consisted of two stages: the first stage, involved the surgical induction of complete tibial fracture under the effect of general anesthesia. The second stages, cast was applied only in G1, while in G2, cast was applied after injection of insulin in the site of fracture, as well as cast was applied after inject PRP at the site of fracture in G3. ultrasonography, computed tomography and histopathology were used  to evaluate the rate of bone fracture healing for each group. The result showed that, the echogenicity change at the fracture rapidly in PRP group followed by insulin group and slowly in control group. The bone density was increased rapidly and high in PRP group followed by insulin group and finally the bone density is low in control group and the histopathological evaluation confirms these results. In conclusion, using of PRP has a greater useful effect in promoting bone fracture healing rate and tissue proliferation than insulin.