A total of 90 random samples of raw cow''s milk used in  small scale ice cream , small scale ice cream and large scale plain ice cream(30 samples each) collected fromAssiut city, Egypt , in the period between  from April and September 2019. Each sample was put in ice tank with thermometer to maintain temp. at 4C. The samples were dispatched to the laboratory with a minimum of delay where they were examined for coliforms, fecal coliforms and E.coli true faecal type.The results show that the mean counts of total coliforms in raw cow''s milk,  small scale and large scale plain ice cream  were 2.4x10³, 2.8x10²  and  1.56x10²  cfu/ml or g. , respectively. While the mean counts of fecal  coliforms were 2.1x10³ , 1.9x10²  and 1.32 x10²   cfu/ml or g. , respectively and  the mean counts of E.coli true faecal type count cfu/ml or g. were 2.5x10² , 3.3x10² and 1.12x10²  cfu/mlor g., respectively.The heath hazards of coliforms, fecal coliforms and E.coli true faecal type, methods of contamination and control werediscussed.