Q fever is an important public health concern throughout the world and infection can lifelong illness in the host. It is caused by the bacterium Coxiella burnetii. The most frequent source of infection for human is domestic ruminants. This survey was carried out from April 2017 to May 2018 in some governorates in Egypt. A total of 1200 samples; 740 blood samples and 405 milk samples were collected from sheep and goat flocks also 55 serum samples were taken from workers of the farms. The overall seroprevalence of C.burnetii in serum and milk was detected in 25.5% & 22.7% and 23.1% & 23.7% in sheep and goats, respectively. While the seroprevalence of Coxiella burnetii in human in this study was 54.5%. Sera and milk were screened using indirect fluorescent technique for detection of IgM & IgG and a Commercial Q fever antibody indirect ELISA test kits (IDEXX Laboratories, USA) to detect anti- C. burnetii IgGantibodies.