Aloe vera (AV) has been used in folk medicine for thousands of years, which has led researchers to study its various health benefits. The aim of this study was to evaluate the effect of AV extract on the motor and exploratory activities in normal healthy rats. The open-field test (OFT) was used to provide a quantitative measurement of the animal motor and exploratory activities. The rats were weighed and randomly divided into three groups (six rats in each) identified as: control group (rats received distilled water (DW)), AV 10% group (rats received AV extract 10%), and AV 20% group (rats received AV extract 20%). All rats were administered DW or AV extracts by oral gavage once daily for 2 weeks at constant doses (2 ml/100 g body weight) among groups. The OFT was performed immediately after 2 weeks using LE8811 Actimeter PanLAB device. The results showed that oral consumption 10% and 20% of AV extract slightly reduced rats' global & locomotor activities, stereotyped movements, distance traveled, and time spent in the fast movements in the OFT. Interestingly, oral consumption 10% and 20% of AV extract completely abolished central rats' rearing activity (exploration) and slightly increased resting time. Additionally, oral consumption of 10% and 20% of AV extract markedly reduced central permanence time and number of entries to center of the arena. However, only oral consumption of 10% AV extract markedly reduced latency to first entry to center of the arena. Under our experimental conditions, these findings lead to the conclusion that AV extract possesses mild sedative and anxiolytic activities in concentration-dependent manner. Further studies are needed to assess these findings using different doses/concentrations of AV extract and other behavioral tests in rats.