Pasteurella multocida (P. multocida) type A is the etiologic agent of fowl cholera, a highly contagious and fatal disease of chickens. In this study 300 birds were examined for isolation of P. multocida. Isolation of P. multocida was attempted from the spleen, lung, trachea and bone marrow collected from chickens. The targeted bacteria from the samples were isolated, identified and characterized based on their morphology, Gram staining, cultural & biochemical characters and confirmed by pathogenicity test and Polymerase Chain Reaction (PCR). Among the examined chicken, 12 (4 %) P. multocida were isolated and identified (n= 12/300). The organisms were gram negative, non-spore forming rod, nonmotile, occurring singly or pairs by Gram staining, whereas in Leishman's stain, bipolarity were observed. All the isolates were positive for oxidase and catalase tests, produced indole. In type specific PCR reaction, the organisms were confirmed as P. multocida type A. Disinfectant suspension test used to determine the efficacy of three disinfectants on the isolated strain, only gluteraldehyde- QAC combination product could achieve standard log. reduction of P.multocida after 15 minutes.