Chlamydia abortus is an obligate intracellular Gram-negative and an important pathogenic bacteria, which can infect animals and humans. 312 blood samples were collected from 17 flocks of Awasi sheep in the down-town and province in Hama governorate between February 2019 and January 2020. The total number of sheep in the study flocks were 3715 including 2972 pregnant sheep. Seropositive cases of C. abortus antibodies were found in 5 of 17 study flocks with a percent of 29.41%. The overall prevalence was (36/312) 11.53% as positive to specific antibodies of C. abortus using ELISA test, which ranged between (0-100%) in tested samples from evry flock in Hama governorate and the seroprevalence in downtown was 14.285%, 16.666 in Koumhana, 33.333% in Almbarkat and 33.333% in Tal Altut, and 100% in Maar Shoor. The higher prevalence of OEA was reported in the winter season comparing with other Seasons (OR= 2.52) and the riskiest ages in OEA were ages between 2 – 4 years.