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168459

MOLECULAR DETECTION OF ENTEROTOXIGENIC GENES FOR STAPHYLOCOCCUS AUREUS ORGANISM ISOLATED FROM RAW MILK AND SOME MILK PRODUCTS

Article

Last updated: 23 Jan 2023

Subjects

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Tags

Food biotechnology
Laboratory investigation
Microbiology, mycology and immunology
Milk hygiene & milk product
Molecular biology

Abstract

This study aimed to determine Staph. aureus in raw milk and some milk products and study the correlation between Staph. aureus enterotoxin genes and its ability to resist different types of antibiotics. A total of 120 raw milk, kareish cheese and baladi yoghurt (40 samples, each) were collected from different dairy shops and street peddlers in Assiut city, Egypt and were bacteriologically examined for presence and count of Staph. aureus. The incidences of counted Staph. aureus in raw milk, kareish cheese and baladi yoghurt were 62.5, 27.5 and 0.0%, respectively, with average counts of 3.25 log, 4.13 log and < 1 log cfu/ml, respectively. All the isolated Staph. aureus strains were tested by Multiplex PCR assay for the presence of enterotoxigenic sea, seb, sec and sed genes and 94.44% of the tested strains harbored sea gene and 2.77% were positive for sed gene, while none of the tested strains was positive for seb and sec gene. The recovered organisms exhibited 52.78, 11.11, 77.78, 61.11, 11.11, 36.11, 63.89 and 16.67% resistance towards Oxacillin (Methicillin), Vancomycin, Amoxicillin, Ceftriaxone, Gentamicin, Tetracycline, Erythromycin, and Trimethoprim-Sulfamethoxazole, while, they exhibited 100% sensitivity towards Ciprofloxacin. The tested organisms showed multi-antibiotics resistance percentage of 55.56% and with average resistance index of 0.37. The correlation between Staph. aureus enterotoxin genes and its ability to resist different types of antibiotics revealed that, most of the enterotoxigenic strains were multi-antibiotics resistance and resist simultaneously to Amoxicillin, Ceftriaxone and Erythromycin. All the methicillin resistant Staph. aureus (MRSA) isolates harbored sea gene. The public health hazards of Staph. aureus in milk and its products as well as the suggestive control measures were discussed.

DOI

10.21608/avmj.2020.168459

Keywords

Staphylococcus aureus, enterotoxigenic genes, Raw milk, kareish cheese, yoghurt, PCR

Authors

First Name

O.A.

Last Name

SADEK

MiddleName

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Affiliation

Food Hygiene Department, Assiut Lab., Animal Health Research Institute, ARC, Egypt

Email

onsi_2000@yahoo.com

City

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Orcid

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First Name

A.M.

Last Name

KORIEM

MiddleName

-

Affiliation

Bacteriology Department, Assiut Lab., Animal Health Research Institute, ARC, Egypt.

Email

-

City

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Orcid

-

Volume

66

Article Issue

167

Related Issue

24252

Issue Date

2020-10-01

Receive Date

2020-09-14

Publish Date

2020-10-01

Page Start

48

Page End

61

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_168459.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=168459

Order

5

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

23 Jan 2023