Ascites in poultry is a serious problem for the commercial broiler industry causing great economic losses. In the present study, the prevalence of ascites was studied in 200 broiler chickens. Also, the correlation between ascites and other systemic lesions at the same bird was investigated. Moreover, identification of the causative bacterial agents was conducted focusing on E. coli and Salmonellae isolates. The prevalence rate of ascites in examined broiler chickens was 17%. Ascites without systemic lesion was observed in 10% of birds while 7% of birds had ascites associated with other systemic lesions in the internal organs and hepatitis was the most frequent lesion. The bacteriological examination revealed that out of 49 samples collected, a total of 40 bacterial isolates were recovered (1.6%). Among the recovered isolates, E. coli was the most prevalent isolate (n=19; 47.5%) followed by Salmonella spp. (n=10; 25%), Proteus species (n=8; 20%) and Enterococcus faecalis (n=3; 7.5%). Antibiogram of E. coli isolates showed a high sensitivity against colistin sulphate while they were highly resistant to the other antimicrobials. Meanwhile, Salmonella isolates showed high sensitivities to ciprofloxacin and enrofloxacin while they were highly resistant to the other antimicrobials.