The rabbit hepatitis E  (rHEV) as been found in farmed, wild and pet rabbits as well as in human patients suggesting zoonotic transmission. The aim of this study was to evaluate the prevalence of HEV-infection in farmed rabbits from hyperendemic regions. Serum anti-HEV was tested by ELISA. We cоllected blоod samples from 235 rabbits from different governоrate in Egypt. HEV RNA was tested using RT-PCR with degenerative primers to ORF2 in serum and feces samples from 235 and 323 farmed rabbits respectively at different governorates in Egypt. All animals were 2-12 months of age. Out оf the 235 rabbit serum samples, the total positive rate of anti‐HEV IgG was 28/235 (11.9%). Anti-HEV IgG prevalence in rabbits was demonstrated by EIA in serum samples in different governorates 13.20%, 13.6 %, 14.6%, 24.2%, 20.0 % in Luxor, Assiut, Fayoum, Menoufia, and Alexandria respectively, and not detected in Sohag and Qena governorates. HEV RNA was detected in serum and fecal samples, only 2 serum samples were positive for HEV RNA 2/235 (0.8 %) at Luxor and Menoufia governorates. Out of the 323 fecal samples, the total positive rate HEV RNA was 26/323 (8.0%). Prevalence of HEV RNA in fecal samples of rabbits varied in different studied governorates from 1.92% to 18.36%, in Qena and Menoufia governorates respectively. HEV prevalence peaked in different age groups at different farms, with majority of infections at age low 6 months. Rabbit HEV infection in Egypt was first documented in our study