Four hundred and fifty (450) samples were collected, 200 from internal organs (liver, caecum, gall bladder and spleen) of 50 ducks, 200 from internal organs of 50 ducklings and 50 unhatched duck eggs (dead in shell embryo) from different farms and hatcheries in Dakahlia Province. All samples were cultured on specific media for isolation and serological identification of Salmonellae spp. A total of 71 samples were positive to Salmonella with total percentage (15.8%). 29, 37 and 5 with (14.5%, 18.5% and 10%) positive samples were isolated from ducks, ducklings and duck eggs, respectively. Serotyping of the isolated Salmonellae from ducklings were five S.infantis, four S.kentucky, ten S.typhimurium, six S.newport, six S.enteritidis and six untypeable Salmonella with a percentage of (13.5%), (10.8%), (27 %), (16.2%), (16.2%) and (16.2%), respectively. Three S. derby, six S.typhimurium, five S.kentucky, three S. shubra, four S. enteritidis, three S.newport and five untypeable Salmonella were isolated from ducks with a percentage of (10.3%), (20.7%), (17.2%), (10.3%), (13.8%), (10.3%) and (17.2%), respectively. On the other hand only one of each S.typhimurium, S.enteritidis, S. newport, S. infantisand untypeable Salmonella were isolated from duck eggs with a percentage of (20%) for each one of these isolates. S.typhimuriumwas the most prevalence isolates and showed high resistance to streptomycin (100%), lincomycin (88%), erythromycin (82%), oxytetracycline (76%) and trimethoprim-sulfamethoxazole (70%). Moderate resistance to neomycin (59%), gentamycin (59%), amoxicillin (53%), ampicillin (53%) and chloramphenicol (47%) we reported. Meanwhile, the lowest resistances were against doxycycline (41%), flumequine (35%), norfloxacin (18%), ciprofloxxacin (6%) and colistin (6%). Conventional PCR assay detected virulence genes (invA, mgtC, sop < /em>B and bcfC) using specific primer for each gene in all examined S.typhimurium samples. This study highlights that, there was significant association between invA, mgtC, sop < /em>B and bcfC virulence genes and pathogenicity of S.typhimurium which showed high multiple antibiotic resistance which require strict regulations of the use of antibiotics in duck farms to minimize the resistant bacterial strains.