This study was carried out on 180, 1-day old, unsexed broiler chicks to evaluate the effect of propolis on growth and immunological parameters, and some biochemical parameters. Chicks were divided into 6 equal groups with 30 chicks in each group and each subgroup was allotted into 5 replicates (6 each) in a complete randomized design. Dietary treatments were as follows: control (without supplementation), propolis at levels of 100,200,300 and 400 mg/kg diet and Tylosin at level 100 mg/kg diet (as a positive control). Results showed that, addition of propolis in the experimental diets increased body weight, body weight gain. Chicks fed diet supplemented with propolis were significantly lower in their feed consumption. While chicks fed control diet consumed the highest amount of feed. The best feed conversion ratio was recorded for chicks fed on 300 mg propolis. Propolis caused significant (P ≤ 0.01and 0.001) decreases in red blood cells (RBC) counts, Packed cell volume (PCV) and hemoglobin (Hb). While a nonsignificant increase in white blood cells (WBCs) counts was detected. Serum total lipids, cholesterol, high density lipoprotein and triglycerides significantly (P ≤ 0.01) decreased with inclusion of different levels of propolis and Tylosin as compared to the control group. Significant (P ≤ 0.001) increase in serum total antioxidant capacity and significant (P ≤ 0.05) decrease in MDA in comparison with the control group. These results means that dietary supplementation by propolis might be useful in productive parameters and can be used as alternative antibiotic in broiler diets.