A field experiment was performed in Gemmiza Agricultural Research Station in 2013 and 2014 seasons and Nubaria Agricultural Research Stations in 2014 season to study the effect of manure and sulfur applications on maize grain yield and other traits. The results showed that the differences due to manure application significantly affected grain yield, plant height and kernels weight. The effect of manure application significantly increased grain yield, where both 10 and 20 m3 of manure treatments gave the highest grain yield at Gemmiza in 2013 season (30.2, 30.5 ardab/fed.), and Nubaria in 2014 season, (31.1 and 33.3 ardab/fed), respectively. Manure application significantly affected on kernels weight, where 20 m3 manure treatment gave the highest value of 100-kernel weight (39.7 g) followed by 10 m3 manure (37.1 g) at Gemmiza in 2013 season. It was noticed that 20 m3 manure treatment gave the highest value of ear length. The application of 10 and 20 m3 manure gave significantly the tallest plants. Significant differences were detected among sulfur application for grain yield. Sulfur application of 200 kg/fed gave more significant means of grain yield. Mean grain yield at Gemmiza in 2013 season for 200 kg/fed sulfur was 32.2 ard/fed compared with 28.5 ard/fad for 100 kg/fed sulfur treatment. At 2014 season, 200 kg/fed sulfur treatment gave 26.2 and 33.6 ard/fed at Gemmiza and Nubaria, respectively. Sulfur application of 200 kg/fed increase ear length with values of 22.2 cm compared with 21.2 and 21.7 cm for the other treatments (100 kg/fed and non sulfur) at Nubaria in 2014 season. Treatment of 200 kg sulfur had the tallest plants in 2014 season with values of 225.0 and 220.2 cm at Gemmiza and Nubaria, respectively. Manure-by-sulfur interaction significantly affected 100-kernel weight at Gemmiza location in 2013 and 2014 seasons, ear length and ear height at Nubaria location in 2014 season. Generally, manure and sulfur application increased 100-kernel weight at 2013 and 2014 seasons. Also, 20 m3 manure with 200 kg sulfur treatment gave significantly the highest value of ear length at Nubaria region in 2014 season (22.7 g).