Wheat (Triticum aestivum L.) is one of the world's major cereal crops. The present work was carried out at the Faculty of Agriculture, (Saba Basha), Alexandria University, Egypt during the seasons of 2014 up to 2016 to study the implementation of specific markers on some major traits in Egyptian wheat. Five Egyptian bread wheat genotypes (2n=42, AABBDD), Egypt 1, Gemmeiza 9 & 11, Sakha 93, Sids 1 and one wild wheat Aegilops ventricosa Tausch (2n=28, DvDvNvNv) were used in the current experiment. Specific peaks for target genes were scored across all genotypes in addition to other unique peaks for susceptible genotypes of all the traits. Polymorphic level was (90%) across all wheat accessions, especially between wild and domesticated wheat. High level of polymorphism could be attributed to selection of genotypes with diverse characteristics. A total of 110 alleles were detected, among which 21 alleles (19%) were polymorphic and 89 alleles were specific for target genes (81%). For SSR, it is very common that each primer set amplifies multiple fragments and they are either different alleles in one locus or different loci. The result reveals significant differences in allelic diversity among wheat cultivars studied. The fragment sizes ranged from 103 to 440bp. This opens up a possibility to apply marker-assisted selection (MAS) in developing new Egyptian wheat cultivars.