To investigate the response of two maize hybrids to spatial distribution and nitrogen fertilization rates. In this respect, two filed experiments were conducted at the Experimental Farm, Faculty of Agriculture (Saba Basha), Alexandria University during 2014 and 2015 seasons in a split- split plot design. Whereas, three factors can be illustrated as follows: the main plot included two maize hybrids (30N11 and 31G98), while, plant spacing (20, 30 and 40 cm) was arranged in the sub plots, while nitrogen fertilization (192, 288 and 384 kg N/ha.) allocated in sub- sub plot. The obtained results cleared that maize hybrid 30N11 recorded higher plant height (cm), ear weight (g), grain weight/ear (g), number of grains /ear, 1000-grains weight (g), number of rows/ear, grain yield (t/ha), biological yield (t/ha), harvest index (%), grains NPK and protein contents than the other hybrid 31G98 in the first and second seasons, respectively. The highest means values ofyield and chemical composition characters wereobtained using nitrogen fertilizer at rate of 384 kg/ha., in both seasons, while the lowestones were recorded by application of nitrogen at 192 kg/ha., in both seasons. Wider spacing between plants (40 cm) produced the higher yield and its components and protein content and NPK in the two successive seasons than narrower spacing (20 cm) which produced the lowest mean values of these characters.