Two filed experiments were carried out at the Experimental Farm of the Faculty of Agriculture (Saba Basha) at Abees region, Alexandria University, Alexandria, Egypt, during the two growing seasons 2014 and 2015 to study the effect of nitrogenous fertilization, A- mycorrhizal inoculation, and effective microorganism (EM) on sunflower cv. Sakha 53 productivity and its oil quality. Experimental design was split- plotwith three replicates. The main results could be summarized as follows: 1) The application of nitrogen fertilizer at 96 kg N/ha., significantly increased stem diameter, head diameter, head weight, number of seeds/head, 100-seed weight, seed yield (ton/ha), straw yield (ton/ha.), biological yield (ton/ha.), Harvest index (%) and Oil yield (ton/ha.), 2) A-Mycorrhizal inoculation + foliar application of EM biofertilizer, significantly, increased yield and its components, as well as, oil yield (ton/ha.), chemical composition (N, P and K) and crude protein content in both seasons, 3) interaction between nitrogen fertilizer of 96 kg/ha and A-mycorrhizae + EM gave the highest values of all yield and its components compared and control treatment (without N fertilizer) with control (without biofertilizer) in both seasons. Also, oil yield (ton/ha.), chemical composition (N, P and K) and crude protein was, significantly, increased due to application 96 kg/ha. + A-mycorrhizae + EM. The present investigation suggest the need for more studies concerning the effect of mineral and biofertilizer, as well as, applying NPK on sunflower plants under different environmental conditions using different types of soils especially newly reclaimed soil, to reach the optimum combination of mineral and biofertilizer to achieve the highest yield and quality of seed oil content.