Two filed experiments were conducted at the Experimental Station Farm of Faculty of Agriculture (Saba Basha), Alexandria University during 2014 and 2015 seasons. The objective of this study was to investigate the response of sesame plants productivity and seed quality to different fertilization methods. The results could be summarized as follows: Foliar spraying of  amino acid gave higher plant height, number of pods/plant, weight of pods (g), number of seeds/pods, 1000-seed weight (g), seed yield (g/plant), seed, straw and biological yields (ton/ha), as well as, oil yield (t/ha) in both seasons. Whereas, phosphorus fertilizer at 30 unit resulted in a significant increment in yield components and chemical composition of sesame seed in both seasons. Significant variation was recorded between the tested biofertilizer treatments for yield, yield components and percentages of oil, N, P and K of sesame seeds in both seasons. The effective treatments for all characters and chemical composition were obtained from foliar spraying of amino acid with phosphorein inoculation in both seasons. The effective treatments for oil percentage, phosphorus percentage and potassium percentage in both seasons were obtained from applying phosphorus at 30 units with phosphorein inoculation.